The Basic Principles Of attune flow cytometer
The Basic Principles Of attune flow cytometer
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Data-enriched characterization of cell populations. Morphological information and facts from pictures can increase to the richness of flow cytometry info. Such as, the figure displays an otherwise common apoptosis assay utilizing Annexin V and PI, incorporating cell imaging to characterize cells in Each individual inhabitants to reveal morphologically unique options. These insights couldn't have already been gained from multiplex staining alone.
As samples are acquired over the Attune CytPix Flow Cytometer, the significant-velocity brightfield digital camera captures and stores illustrations or photos of detected occasions in a charge approximately six,000 illustrations or photos per next, according to the flow fee and picture size. For bigger overall flexibility, Attune Cytometric Software helps you to adjust the image seize frequency as desired and choose a specific gate for impression seize, if demanded.
Blend acoustic focusing technologies with high-parameter Investigation for adaptable spectral unmixing and standard workflows.
Find new insights into your experimental details by visualizing populations using combined fluorescence and morphology information. Our software program interface wizards allow downsampling, concatenation (merged information) and dimensionality resources which might be crafted into your application for an intuitive, streamlined information Assessment working experience.
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Furthermore, it's illuminating to check out how many doublets slip as a result of conventional singlet gates, and good to confirm the status of unpredicted double-beneficial occasions in my analysis."
Optimizing gating tactic. Even robust guide singlet gating is error-inclined and continues to be a subjective determination level in Virtually all flow cytometry assays. Imaging may be used to confirm and adjust gates to incorporate only solitary cells of interest.
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Early signs of this contamination experienced originally been dismissed as debris. Backgating to find new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also lets you use morphological functions to find fascinating subpopulations that would not be obvious from flow cytometry info alone.
Possibly most importantly, these functions consist of cells of clearly different phenotypes which could lead to incorrect conclusions concerning double good gatherings (especially in scarce populations).
Audio waves help exact cell alignment, resulting in Remarkable info good quality and ~10X more quickly sample flow rates.
Application Be aware: Automatic impression Evaluation decreases consumer-to-consumer variability in flow cytometry gating procedures
Developed-in dimensionality reduction resources increased resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to substantiate expected populace resolution in lysed full human blood. Person can check out events from Every cluster for visual confirmation of mobile phenotypes.
No wash/no lyse leukocyte analysis with imaging. Cells were obtained from 24-hour-previous blood by dilution in 1 mM EDTA ( Expert UX/UI allows people to teach AI models for expanded applications. (A) The “Coach Design” function supplies annotation applications and genuine-time model education precision.
Gating on these extended parameters allows you to immediately and precisely identify populations of interest and make sure gating technique with little or no guide evaluate. The Attune software features usage of condition-of-the-art equipment Finding out Evaluation resources within just an expertly intended consumer interface, rendering it extremely convenient to use.
Since the mobile line had Beforehand been analyzed on the Attune CytPix Flow Cytometer, the researchers went back again to the photographs and have been capable of document the microbial an infection at least 5 days earlier.
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What seemed to be a homogeneous inhabitants is in fact far more diverse—an insight that needs to be regarded as when interpreting benefits.